Fig. 3: Single-cell RNA sequencing analysis on CiNs in the striatum.
From: In vivo chemical reprogramming of astrocytes into neurons
a Representative images of neuron-like EGFP+ cells in brain slices collected by patch pipette. Scale bar, 10 μm. b EGFP expression was confirmed in the 51 cells analyzed. c t-SNE analysis of highly variable genes that were detected in 51 EGFP+ cells. d Heatmap representation of 1256 differentially expressed genes cross the three clusters. e Enriched GO terms of 1256 differentially expressed genes cross the three clusters. f Gene expression profiles of the EGFP+ cells using the neuron score analysis. g Expression patterns of striatal neuronal subtype genes in the three clusters. h A heatmap showing expression levels of genes involved in mature neuronal functions in the three clusters. i A heatmap showing expression levels of GABAergic neuronal genes in the three clusters.
