Fig. 2: Detection of intraocular bacteria in AH specimens and cultures.

a Negative staining transmission electron microscopy was used to visualize cultured P. acnes at 20,000× magnification. The negative control shows the visualization of water without AH specimen. b Negative staining transmission electron microscopy shows bacteria in minimally manipulated fresh AH specimens at 20,000× magnification. c Cultured E. coli was visualized by light microscopy. The negative control consists of sample preparation buffer without any AH inoculation. d Bacteria in cultured AH samples (examples of culture-positive and -negative samples) were visualized by light microscopy. e Representative anaerobic cultures of skin specimens and negative controls (PBS solution used to dilute specimens). f Representative anaerobic cultures of AH specimens.