Fig. 3: Validation of the function of CspL in vitro. | Cell Discovery

Fig. 3: Validation of the function of CspL in vitro.

From: A cold shock protein promotes high-temperature microbial growth through binding to diverse RNA species

Fig. 3: Validation of the function of CspL in vitro.

a Binding of variously-sized RNA and ssDNA fragments to CspL. The vertical red line separates the association and disassociation steps. An absolute binding value <0.05 nm indicates no binding signal. b Bio Layer Interferometer (BLI) for testing seven conserved amino acid-mutations. c, d The growth curves (c) and DCWs (d) of mutants compared with the WT and the empty vector control. ** P < 0.01 and ***P < 0.001 (two-tailed Student’s t-test).

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