Fig. 4: TAZ is required for chondroprogenitor cell proliferation.
From: TAZ is required for chondrogenesis and skeletal development
a Cell proliferation rate of chondrocytes from Col2-Cre;TAZf/f mice and controls as shown by WST-1 assay after D0, D1, D2, and D3. b WST-1 assay of chondrocytes with shTAZ lentivirus for different periods of time as indicated. c WST-1 assay of chondrocytes with TAZ overexpression for different periods of time as indicated. d Colony formation assay using primary chondrocytes from Col2-Cre;TAZf/f mice and controls. The corresponding quantitative analysis was performed at right. e Fluorescence images of BrdU+ in the tibiae from Col2-Cre;TAZf/f mice and controls at P10. The BrdU+ cells are quantified in the corresponding column at right. Scale bars, 75 μm. f TUNEL staining and quantitative analysis of the apoptosis-positive cell in the region of the articular cartilage at age of 1-month Col2-Cre;TAZf/f mice and controls. The apoptosis-positive cells are quantified in the corresponding column at right. Scale bars, 75 μm. The experiment was independently repeated for three times. Error bars were the means ± SEM of triplicates from a representative experiment. *P < 0.05, **P < 0.01, ***P < 0.001.
