Fig. 7: Deletion of TAZ in chondrocytes impaired the fracture healing.
From: TAZ is required for chondrogenesis and skeletal development
a Representative X-ray images of the fractured femora of 3-month-old Col2-Cre;TAZf/f mice and controls after 2-week fracture injury on the left hindlimbs. The white arrows point to the fracture site. n = 5 mice per group. b Representative micro-CT reconstructions of the callus tissues at the fracture sites after 2-week surgery (n = 5 mice per group). Scale bars, 1 mm. c Histomorphometric analysis of bone parameters from b. Bone volume fraction (BV/TV); trabecular thickness (Tb.Th); trabecular number (Tb.N); trabecular spacing (Tb.Sp). d Representative Alcian blue staining image of the fracture calluses after 2-week injury. Scale bars, 1000 μm (left panel); 50 μm (right panel). e Alcian blue-positive area and intensity were quantified by ImageJ software based on Alcian blue staining in d. f Gene expression analysis of the chondrocyte markers using tissues isolated from fracture callus. g, h Immunofluorescence staining analysis for Col10a1 and Runx2 in fracture callus tissues from Col2-Cre;TAZf/f mice and controls. Scale bars, 50 μm. The experiment was independently repeated for three times. Error bars were the means ± SEM of triplicates from a representative experiment. *P < 0.05, **P < 0.01.
