Fig. 1: Infectivity analyses of the coronaviruses.

a SARS-CoV-2, PCoV-GD, PCoV-GX, and RaTG13 pseudotyped viruses were used to infect a total of 26 cell lines. The infected cell lysates were analyzed for relative light units (RLU). All results were obtained from six independent experiments (mean ± SEM). In each experiment, the infection assay was performed in duplicate wells. The average value of the duplicate wells was presented in the plot as a circle. The one-way ANOVA and Dunnett’s multiple comparisons test were employed to study the differences of infectivity between SARS-CoV-2 and the other three coronaviruses. When the RLU for the tested cell reached 1% of that for 293T-hACE2 cell, it was deemed as a susceptible cell line. The dashed line indicates the 1% RLU value of the 293T-hACE2 cell. b The infections of SARS-CoV-2, PCoV-GD, PCoV-GX, and RaTG13 pseudotyped viruses to 293T-hACE2 could be inhibited by soluble hACE2. All results were obtained from six independent experiments (mean ± SEM). In each experiment, the infection assay was performed in duplicate wells. c The relative infectivity of the pseudotyped viruses with mutant S protein was presented as the ratio of RLU for mutants/RLU for original ones. All results were obtained from two independent experiments (mean ± SEM). In each experiment, the infection assay was performed in duplicate wells.