Fig. 4: Characterization of the mitochondrial distribution in epididymal regions. | Cell Discovery

Fig. 4: Characterization of the mitochondrial distribution in epididymal regions.

From: Spatio-temporal landscape of mouse epididymal cells and specific mitochondria-rich segments defined by large-scale single-cell RNA-seq

Fig. 4: Characterization of the mitochondrial distribution in epididymal regions.The alternative text for this image may have been generated using AI.

a The segmental percentage of mitochondrial transcripts (MTs) in epididymal epithelial cell clusters. b Stitched image of immunostaining of the mitochondrial marker cytochrome c in the mouse epididymis. Scale bar = 1 mm. c Representative images of cytochrome c staining in the caput, corpus and cauda epididymis. Scale bar = 50 µm. Red: cytochrome c (cyto C), Blue: DAPI for nuclear count staining. d Corresponding statistics of cytochrome c staining in the segmental epididymis. **P < 0.01; ***P < 0.001. e UMAP plots of mitochondrial transcript genes (mt-cytb and mt-Nd1) in the indicated cell clusters (color values were log1pRPM). f Representative mitochondrial transcript genes (mt-cytb and mt-Nd1) in sperm-depleted epididymal segments by qPCR analysis. The statistical significance was corpus or cauda vs. caput. ****P < 0.00001.

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