Fig. 1: Overview of the human SETD2–hNCPH3.3K36M complex structure.

a Schematic drawing of the domain organizations of human SETD2. The color scheme is the same as that of the SETD2–hNCPH3.3K36M structural model. b Cryo-EM density map of human SETD2–hNCPH3.3K36M complex at 3.1 Å shown from two orthogonal views. The cryo-EM map was segmented and colored according to the respective components of the SETD2–hNCPH3.3K36M complex. c Atomic model of human SETD2–hNCPH3.3K36M complex shown from two orthogonal views. The catalytic domain of SETD2 binds to the α-N of histone H3.3 as well as the nucleosome DNA at SHL1.