Fig. 3: Histone H1 represses the deposition of H3K36me3 by SETD2. | Cell Discovery

Fig. 3: Histone H1 represses the deposition of H3K36me3 by SETD2.

From: Cryo-EM structure of SETD2/Set2 methyltransferase bound to a nucleosome containing oncohistone mutations

Fig. 3

a Schematic drawing of the nucleosome bound with a linker histone H1. b HMT assays performed with SETD2 toward the 197-bp nucleosomes bound with histone H1.4. The input of the HMT reactions is shown in Supplementary Fig. S5a. c Integrative Genomics Viewer (IGV) tracks representing the distributions of H3K36me3, H1.4 and IgG ChIP-Seq results with two replicates. Rep1, replicate 1, Rep2, replicate 2. d Venn diagram illustration showing the overlap of H3K36me3, H1.4 and IgG peaks. The peaks in independent replicates were combined. e The percentages of H3K36me3, H1.4 and IgG peaks in different genomic elements. ChIP-Seq results in independent replicates were combined. The distributions were analyzed by HOMER with default parameters. f The enrichments of H3K36me3, H1.4 and IgG ChIP-Seq results at H1.4 peak regions. A 100-bp sliding window was used to scan 10-kb regions surrounding the peak center. Heatmaps illustrating ChIP-Seq read densities from 5 kb upstream to 5 kb downstream of the peak regions (in rows) were shown on a per-peak basis (in columns). g The distribution profiles of normalized H3K36me3, H1.4 and IgG read density from 5 kb upstream of the TSS to 5 kb downstream of the TES.

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