Fig. 7: Reprogramming of sRNomes during hepatic reprogramming.

a Schematic illustration of the strategy used for direct conversion of human MSC^LT to hepatocytes. b Fluorescent immunostaining of MSC^LT and MSC^LT infected with FOXA3, HNF1A, and HNF4A (FHH) for 14 days. Scale bar: 100 μm. c Distribution of different types of sRNAs in PHH and MSC^LT infected with FHH. d Hierarchical clustering of unique reads (RPM > 20 in at least one sample) from sequenced samples showing the decreased expressions of MSC^LT-enriched sRNAs and increased expression of PHH-enriched sRNAs during hepatic reprogramming. e Differential expression levels of U1-5′snsRNA in MSC^LT and PHH. The expression levels of U1-5′snsRNA gradually decreased in MSC^LT cells infected with FHH. f Treatment of U1-5′ ASO promoted the induction of hepatic marker genes in MSC^LT cells overexpressing FHH.