Fig. 3: Distribution and function of BudA and AdhE in bacteria. | Cell Discovery

Fig. 3: Distribution and function of BudA and AdhE in bacteria.

From: 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism

Fig. 3

a The Venn diagram illustrates the occurrence and overlap of BudA and AdhE through genome context analysis. A total number of 4333 genomes were obtained by NCBI Blast online with the restriction of maximum identity level higher than 50%, query coverage of more than 90%, and E value lower than e−30. b Organizations of 2,3-BD biosynthesis operon and adhE gene in different bacteria. c Growth of K. pneumoniae ATCC 25955 using glucose as carbon source. d Growth of K. pneumoniae ATCC 25955 (ΔadhE) using glucose as carbon source. e Growth of K. pneumoniae ATCC 25955 (ΔbudABC) using glucose as carbon source. f Growth of E. coli K12 using glucose as carbon source. g Growth of E. coli K12-pETRABC using glucose as carbon source. h Growth of E. coli K12 (ΔadhE)-pETRABC using glucose as carbon source. Experiments were carried out under aerobic conditions. Data shown are means ± SD (n = 3 independent experiments).

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