Fig. 4: Pta-AckA rather than AcsA is essential in the biotransformation of acetate to acetyl-CoA during acetate elimination. | Cell Discovery

Fig. 4: Pta-AckA rather than AcsA is essential in the biotransformation of acetate to acetyl-CoA during acetate elimination.

From: 2,3-Butanediol synthesis from glucose supplies NADH for elimination of toxic acetate produced during overflow metabolism

Fig. 4

a Concentrations of 13C-labeled acetate in E. cloacae SDM and its derivatives. b Concentrations of 13C-labeled ethanol in E. cloacae SDM and its derivatives. 13C sodium acetate at a concentration of about 10 mM was added into the culture system of strains at 2 h. After 4 h of culture, the concentrations of 13C ethanol and 13C acetate were assayed. Experiments were carried out under aerobic conditions. Data shown are means ± SD (n = 3 independent experiments). ***P < 0.001 in two-tailed Student’s t-test; ns, no significant difference (P ≥ 0.05 in two-tailed Student’s t-test).

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