Fig. 6: TSCs transplantation improves oocyte development and quality.

a Statistics on the number of oocytes obtained for superovulation after TSCs transplantation. b Phase-contrast micrographs of oocytes in different groups. The green arrow represents MII, the blue arrow represents MI, and the yellow arrow represents GV. The number of oocytes obtained after COS. c Statistics on the maturation percentage of oocytes obtained for superovulation after TSCs transplantation. d The quality of eggs was observed by immunofluorescence staining with an anti-cleaved caspase3, TOM-20, and ROS in the oocytes. e Statistics analysis of cleaved caspase3 fluorescence indicating oocytes apoptosis, the density of fluorescence represents the level of apoptosis. f The richness of mitochondria in oocytes was observed by immunofluorescence staining with an anti-TOM20, and the density of fluorescence represents the number of mitochondria. g Statistics analysis of the oxygenation of the oocytes were observed by immunofluorescence staining with an anti-ROS probe, and the density of fluorescence represents the level of ROS. h Chromosomal behavior (anti-β-tubulin and DAPI). i Statistics analysis of the chromosomal behavior in the oocytes. j The percentage of the oocyte fertilization after ICSI in vitro. k The percentage of the blastocyst of the embryo after ICSI in vitro. The CON group represents the normal wild type monkeys (n = 3), the POI group represents the monkeys with premature ovarian failure (n = 3), the ADSCs group represents the monkeys with POI that received adipose-derived mesenchymal stem cells transplantation (ADSCs = POI + ADSCs, n = 3); the TSCs group represents the monkeys with POI that received thecal stem cells transplantation (TSCs = POI + TSCs, n = 3). Data were expressed as the means ± SEM and significance were determined by one-way ANOVA. *P < 0.05, **P < 0.01, ns not significant.