Fig. 6: FadA functions through its acetyltransferase activity. | Cell Discovery

Fig. 6: FadA functions through its acetyltransferase activity.

From: Interception of host fatty acid metabolism by mycobacteria under hypoxia to suppress anti-TB immunity

Fig. 6: FadA functions through its acetyltransferase activity.

a Alignment of H359 and C389 sites of FadA in mycobacteria and bacteria. b In vitro two-substrate steady-state kinetic assay of acetyltransferase activity of recombinant FadA and FadA-Mut (FadA(H359A), FadA(C389A)) protein. c Assay of the acetyl-CoA level of peritoneal macrophages infected with H37Rv, H37RvΔFadA, H37Rv(ΔFadA + FadA), H37Rv(ΔFadA + FadA(H359A)), and H37Rv(ΔFadA + FadA(C389A)) strains for 4 h (MOI = 1) (mean ± SEM). d qPCR analysis of Il6 mRNA of peritoneal macrophages infected with H37Rv, H37RvΔFadA, H37Rv(ΔFadA + FadA), H37Rv(ΔFadA + FadA(H359A)), and H37Rv(ΔFadA + FadA(C389A)) strains for 4 h (MOI = 1) (mean ± SEM). e Adult zebrafish were intraperitoneally infected with roughly 200 CFU of WT, ΔFadA, ΔFadA + FadA, ΔFadA + FadA(H373A), and ΔFadA + FadA(C403A) M. marinum strains for 14 days. Histopathology was assessed in the whole fish by bacterial titers (mean ± SEM of n = 5 fish infected for 14 days). Total number of zebrafish analyzed: five (WT), five (ΔFadA), five (ΔFadA + FadA), five (ΔFadA + FadA(H373A)), five (ΔFadA + FadA(C403A)). Data in b–e represent one experiment with at least three independent replicates. One-way ANOVA with Bonferroni’s multiple comparisons test (c–e) was used for statistical analysis.

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