Fig. 2: WDR63 protein is highly expressed in late stages of spermatogenesis and Wdr63-null males are infertile.
From: Bi-allelic variants in human WDR63 cause male infertility via abnormal inner dynein arms assembly
a Real-time q-PCR for Wdr63 transcripts in various 8-weeks mouse tissues, and Gapdh gene is used as a control. Error bars, SEM (n = 3). b mRNA levels of Wdr63 in mouse testis at the indicated time points. Error bars, SEM (n = 3). c Schematic diagram of CRISPR/Cas9 strategy for the generation of Wdr63 KO mice. The sgRNA was designed to target exon 10 of the Wdr63, a nonsense mutation (GCG-TGA) plus a 2-bp deletion was obtained. d Western blot analysis of WDR63 protein in wild-type (Wdr63+/+), heterozygous (Wdr63+/−) and homozygous (Wdr63−/−) mice with a mouse polyclonal antibody raised against WDR63. β-actin was used as a loading control. e Fertility analysis for adult Wdr63+/+ and Wdr63−/−. f, g The morphology and testis weight of testes and epididymis from Wdr63+/+ and Wdr63−/− males at 8-week-old. Scale bars, 0.5 cm. h Total sperm number was dramatically decreased in Wdr63−/− males compared to that of Wdr63+/+ males. Abbreviations: W, week; M, million. For e to h, n = 10 and the bars represent means ± SEM. The statistical analysis was carried out using One-way ANOVA test, ** denotes P < 0.01; ns, not significant.
