Fig. 1: The impact of OX40L on Th cell polarization depends on the cytokine polarizing context. | Cell Discovery

Fig. 1: The impact of OX40L on Th cell polarization depends on the cytokine polarizing context.

From: A multivariate modeling framework to quantify immune checkpoint context-dependent stimulation on T cells

Fig. 1: The impact of OX40L on Th cell polarization depends on the cytokine polarizing context.

a Experimental design of the Th cell polarization assay. b Heatmap representing OX40L-induced scaled absolute difference on the 17 output cytokines measured in each Th context. The absolute difference corresponds to the concentration of a given cytokine in a Th context in the presence minus absence of rhOX40L. The mean over 13 donors is represented. Paired Wilcoxon’s test was used to evaluate the significance of OX40L’s effect. c IL-10, IL-13, and IL-22 production in each Th context in the presence (red dots) or absence (black dots) of rhOX40L. Data are the means ± SD, and individual values from 13 donors are represented. Paired Wilcoxon’s test was used for statistical analysis. d PCA representing the barycenters of the four Th-polarizing contexts in the presence (red dots) or absence (black dots) of rhOX40L. ns, no significance; *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001.

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