Fig. 1: Identification of Pol III complex as tPARP1-binding partners.
From: Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
a The domain architecture of human PARP1 and its orthologs in a set of lower eukaryotes. Caspase 3 cleavage site on human PARP1 is at D214. b A schematic representation of the tandem affinity purification assay to identify mutant tPARP1-binding partners. c List of the top 11 unique proteins with more than two unique peptides identified from mutant tPARP1 (mtPARP1) purification using mass spectrometry. d Pol III complex was only identified in the protein affinity purification using mtPARP1 and mutant TARG1 (mTARG1) but not from that using mutant PARP1 (mPARP1) or mutant PARG (mPARG).
