Fig. 3: tPARP1 recognizes the Pol III complex during apoptosis.
From: Truncated PARP1 mediates ADP-ribosylation of RNA polymerase III for apoptosis
a The cleavage of PARP1 was examined in a time course in poly(dA-dT)-stimulated (3 μg/mL) apoptotic U2OS cells with anti-PARP1 N-terminus (PARP1) or C-terminus (tPARP1) antibody. b Apoptosis induced by 5 μg/mL of poly(dA-dT) in U2OS cells was determined by Annexin V-FITC/PI double staining. c Morphology change of apoptotic U2OS cells stimulated by 5 μg/mL poly(dA-dT) was recorded every 3 h for a total of 12 h. Scale bar, 50 μm. d mPARP1 was expressed in U2OS PARP1-null cells, followed by 5 μg/mL poly(dA-dT) induction for 6 h. mtPARP1 but not mPARP1 interacted with endogenous POLR3A, POLR3B, or POLR3F. Data are represented as means ± SD as indicated from three independent experiments. Significance of differences was evaluated by Student’s t-test. **P < 0.01; ***P < 0.001.
