Fig. 1: Cross-species transcriptomic features associated with differential regenerative capacities.
From: Cross-species metabolomic analysis identifies uridine as a potent regeneration promoting factor
a Flowchart of experimental design for obtaining transcriptome and metabolome data from samples with differential regenerative capacities: axolotl blastema (AB) at DPA 0 and DPA 11, deer antler stem cells (dASCs), tissues from young and aged non-human primates (NHPs), and young and aged hMSCs. b Left, PCA analysis of the transcriptome data of AB at DPA 0 and DPA 11 (top) and WT and WS hMSCs (bottom). Right, tree plot showing the Euclidean distance for transcriptome data of young and aged NHP tissues. c Circos plots showing the overlap of upregulated (top) and downregulated (bottom) DEGs in AB at DPA 11, young tissues, and young hMSCs. d Bubble plot showing the enriched GO terms and pathways for upregulated DEGs in AB at DPA 11, young tissues, and young hMSCs. The color key from white to amaranth indicates low to high –log10(P-value). e Left, bubble plot showing the relative differential expression (DE) score for all genes and regeneration genes in AB at DPA 11, young tissues, and young hMSCs. The color key from white to amaranth indicates the relative DE scores from low to high. Right, bubble plot showing the convergently upregulated regeneration DEGs in AB at DPA 11, young tissues, and young hMSCs. The color key from white to amaranth indicates log2(fold change) values of DEGs from low to high. Genes convergently upregulated in at least four tissues/cells with high regenerative capacity were shown. f Left, bubble plot showing the relative DE score for all genes, mitochondria-related genes or metabolic genes in AB at DPA 11, young tissues, and young hMSCs. Middle, bubble plot showing the convergently upregulated mitochondria-related DEGs in AB at DPA 11, young tissues, and young hMSCs. Right, bubble plot showing the convergently upregulated metabolic DEGs in AB at DPA 11, young tissues, and young hMSCs. The color key from white to amaranth indicates DE scores (left) or log2(fold change) values of DEGs (middle and right) from low to high. Genes convergently upregulated in at least four tissues/cells with higher regenerative capacity were shown.
