Fig. 5: Uridine promotes cardiac repair after myocardial infarction.

a Schematic diagram for the time course of the mouse myocardial infraction (MI) modeling and vehicle or uridine treatment. Uridine or vehicle treatment were performed every other day, as indicated by the red arrows. b Left, representative echocardiographic images of sham mice (n = 6 mice) and mice treated with vehicle (n = 13 mice) or uridine (n = 13 mice) at day 7 after MI modeling and intramyocardial injection of vehicle or uridine. Right, quantitative data of left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (LVFS). Data are presented as the means ± SEM (two-tailed unpaired Student’s t-test). c Quantitative analysis of LDH and CK level of sham mice (n = 15 mice) and mice treated with vehicle (n = 25 mice) or uridine (n = 25 mice) on the next day after MI modeling and intramyocardial injection of vehicle or uridine. Data are presented as the means ± SEM (two-tailed unpaired Student’s t-test). d Gene set enrichment analysis showing relative expression levels for downregulated DEGs upon myocardial infarction (top) and upregulated DEGs upon myocardial infarction (bottom) in the heart tissues from vehicle- or uridine-treated mice. e Top, Venn diagram showing the downregulated genes upon myocardial infarction and upregulated genes upon uridine treatment as compared to vehicle treatment. The overlapped genes were defined as “rescue DEGs (upregulated)” (left). Ring-heatmap plot showing the relative expression levels of “rescue DEGs (upregulated)” in mouse myocardial infarction model (right). Bottom, Venn diagram showing the upregulated genes upon myocardial infarction and downregulated genes upon uridine treatment as compared to vehicle treatment. The overlapped genes were defined as “rescue DEGs (downregulated)” (left). Ring-heatmap plot showing the relative expression levels of “rescue DEGs (downregulated)” in mouse myocardial infarction model (right). The color key from blue to amaranth indicates log₂(fold change) values from low to high. f Top, GO term and pathway enrichment analysis of “rescue DEGs (upregulated)” in mouse myocardial infarction model. Bottom, GO term and pathway enrichment analysis of “rescue DEGs (downregulated)” in mouse myocardial infarction model. The color keys from white to red or blue indicate –log₁₀(P-value) from low to high.