Fig. 6: Uridine treatment enhances in vivo tissue regeneration and repair.

a Schematic diagram for the experimental design of the mouse liver fibrosis (LF) modeling and vehicle or uridine treatment. b Bar charts of liver weight (left) and liver index (right) of sham mice (n = 10 mice) and liver fibrotic mice treated with vehicle (n = 9 mice) or uridine (n = 10 mice). c Representative images of Masson staining of the liver from sham mice (n = 10 mice) and liver fibrotic mice treated with vehicle (n = 9 mice) or uridine (n = 10 mice). Quantitative data of the relative fibrotic area are shown to the right. Scale bars, 200 μm. d Diagnostic tests for liver functions of sham mice (n = 10 mice) and liver fibrotic mice treated with vehicle (n = 9 mice) or uridine (n = 10 mice). e Schematic diagram for the time course of the mouse hair regeneration experiment. f Hair-growth effect of mice topically treated or subcutaneously injected with vehicle or uridine. Hair-growth rates upon vehicle or uridine treatment were verified by pigmentation scoring. Subcutaneous injection (Vehicle, n = 7 mice; Uridine, n = 8 mice). Topical treatment (Vehicle, n = 6 mice; Uridine, n = 10 mice). g Representative images of H&E staining of the hair follicle of mice subcutaneously injected with vehicle or uridine at day 14 post treatment. Scale bars, 400 μm. h Pie plots showing the hair follicle phase of mice subcutaneously injected with vehicle or uridine at day 14 post treatment. Mean values of hair follicle phases for mice subcutaneously injected with vehicle (n = 7 mice) or uridine (n = 8 mice) are shown. i Ki67 and KRT15 staining of the hair follicle of mice subcutaneously injected with vehicle (n = 7 mice) or uridine (n = 8 mice) at day 14 post treatment. Scale bars, 200 μm. j Schematic diagram for the experimental design of anterior cruciate ligament transection (ACLT) mediated osteoarthritis (OA) modeling and vehicle or uridine treatment. k Representative images of Safranin O/ Fast Green staining of articular cartilage from the joints of sham mice (n = 10 mice) and OA mice treated with vehicle (n = 10 mice) or uridine (n = 10 mice). Quantitative data of cartilage thickness are shown to the right. Scale bars, 100 μm. l Bar chart showing the times of electric shock for sham mice (n = 7 mice) and OA mice treated with vehicle (n = 7 mice) or uridine (n = 7 mice) on the treadmill within 30 min at day 33 post vehicle or uridine treatment. m Bar chart showing the grip strength evaluation of the forelimbs and hind limbs of sham mice (n = 10 mice) and OA mice treated with vehicle (n = 10 mice) or uridine (n = 10 mice) at day 33 post vehicle or uridine treatment. n Schematic diagram of the long-term oral administration experiment. o Bar chart showing the grip strength evaluation of the forelimbs and hind limbs of mice orally administered vehicle (n = 26 mice) or uridine (n = 26 mice) at day 57. p Bar chart showing the times of electric shock for mice orally administered vehicle (n = 26 mice) or uridine (n = 22 mice) on the treadmill within 30 min at day 63 post treatment. Data in b–d, f, i, k–m, o, and p are presented as the means ± SEM. (two-tailed unpaired Student’s t-test).