Fig. 3: Defects in asymmetric actin assembly generated extra Q.p progenies.
From: Wnt signaling polarizes cortical actin polymerization to increase daughter cell asymmetry
a, b Schematics and fluorescence inverted images of the AVM (red arrows) and PVM (blue arrows) neurons derived from Q.p lineages in WT (a) and wve-1 conditional knockout (b) animals. A/PVM neurons were visualized using Pmec-4::gfp. The cell identities are denoted adjacent to the cells; asterisks indicate the co-injected marker. Dotted black lines show the periphery of C. elegans. c Quantification of Q lineages with extra A/PVM neurons in WT (n > 300) and indicated mutant animals. n is as labeled. Statistical significance: *P < 0.05, ***P < 0.001 based on χ2 test. d Representative fluorescence inverted images of the A/PVM neurons in animals with indicated genotypes. e Representative fluorescence inverted images of the A/PVM neurons in abi-1 conditional knockout or its Cas9-cleavage resistant transgene (mabi-1(+)) rescued animals. Scale bar in (a, b) and (d, e) 50 µm.
