Fig. 6: Interaction between Myc upregulation and Tnfaip3 downregulation in microglia. | Cell Discovery

Fig. 6: Interaction between Myc upregulation and Tnfaip3 downregulation in microglia.

From: Distinct phases of adult microglia proliferation: a Myc-mediated early phase and a Tnfaip3-mediated late phase

Fig. 6

a qRT-PCR of sorted lumbar cord microglia. Overexpression of Myc in microglia of adult naive Cx3cr1-creER-yfp/+; RFS-Myc/+ animals resulted in downregulation of Tnfaip3. b qRT-PCR of sorted lumbar cord microglia from Cx3cr1-creER-yfp/+; Myc fl/fl cKO animals. Deletion of Myc from adult microglia did not prevent Tnfaip3 downregulation 2 days after nerve injury. c qRT-PCR of sorted lumbar cord microglia. Deletion of Tnfaip3 from adult microglia prevented Myc upregulation 1 day after nerve injury. d Quantification of Ki67 flow cytometry. The proliferation of lumbar cord microglia in Cx3cr1-creER-yfp/+; Tnfaip3 fl/fl cKO animals did not increase after nerve injury. Analyses are unpaired two-tailed t-test (a, b) and one-way ANOVA with Tukey’s multiple-comparison test (c, d), n = 4–6, means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.

Back to article page