Fig. 7: The termination of microglia proliferation.

a qRT-PCR of sorted lumbar cord microglia from Cx3cr1-creER-yfp/+; +/+ animals. The expression levels of Myc, Cdk1, and Tnfaip3 of lumbar cord microglia returned to control contralateral levels 7 days after nerve injury. b Quantification of Ki67 flow cytometry. Overexpression of Myc in adult microglia or deletion of Tnfaip3 from adult microglia prevented the termination of lumbar cord microglia proliferation 7 days after nerve injury. Analyses are one-way ANOVA with Tukey’s multiple-comparison test (b) or two-way ANOVA with Sidak’s multiple-comparison test (a), n = 4–6, means ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.