Fig. 1: Cell isolation, antibody cloning, and candidate panning. | Cell Discovery

Fig. 1: Cell isolation, antibody cloning, and candidate panning.

From: Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

Fig. 1: Cell isolation, antibody cloning, and candidate panning.The alternative text for this image may have been generated using AI.

a Isolation strategy of highly potent neutralizing antibodies as depicted by a diagram. b RBD-specific B cells were isolated from convalescent subjects of SARS-CoV-2 infection by fluorescence-activated cell sorting. The 7ADD–/CD19+/CD27+/IgG+/RBD+ gate is shown and highlighted in the boxes. c Statistics of the number of paired antibodies from each subject, as well as the number of kappa and lambda subtypes. d Binding scores of antibody candidates against SARS-CoV-2 RBD as measured by ELISA and scores higher than 2 are presented. 2G1 is highlighted in red. e Candidate panning using a WA1/2020 pseudovirus-based screening model. Antibodies were 10-fold serially diluted from 101 μg/mL to 10−4 μg/mL.

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