Fig. 3: Binding, blocking, and extensive neutralization of 2G1 against SARS-CoV-2 variants. | Cell Discovery

Fig. 3: Binding, blocking, and extensive neutralization of 2G1 against SARS-CoV-2 variants.

From: Broad ultra-potent neutralization of SARS-CoV-2 variants by monoclonal antibodies specific to the tip of RBD

Fig. 3: Binding, blocking, and extensive neutralization of 2G1 against SARS-CoV-2 variants.

a, b 2G1 competitively blocked the ACE2 binding to single point mutant RBD proteins (a) and VOC S trimers (b). c Affinity analysis of 2G1 bound to S trimers of SARS-CoV-2 WA1/2020, Alpha, Beta, Gamma, Kappa and Delta by SPR. Chips fixed with S trimers were loaded on a BIAcore 8 K system. 2G1 Fab varied from 1.250 μg/mL to 0.039 μg/mL were injected over the chips for measuring the real-time association and dissociation parameters. d Neutralization of 2G1 against diverse SARS-CoV-2 pseudoviruses. Pseudoviruses with active titer higher than 1 × 107 TU/mL were employed in this study. Concentration-dependent neutralization of 2G1 was quantified by detecting the fluorescence from the luciferase reporter. Data in duplicate are displayed as means ± SD. e Live virus neutralization by 2G1. 100 TCID50 of SARS-CoV-2 (WA1/2020, Alpha, Beta, Gamma and Delta) were incubated with 3 fold-diluted 2G1 and then added to Vero E6 cells. After a 3-day incubation, cytopathic effect (CPE) was assessed by counting the plaque formation.

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