Fig. 3: OsSAPK6 enhances the stability of IPA1.
From: Chilling-induced phosphorylation of IPA1 by OsSAPK6 activates chilling tolerance responses in rice
a Protein levels of IPA1 in ZH11, Ubi:OsSAPK6 and ossapk6-2 2-week-old seedlings. b Expression levels of IPA1 in ZH11, Ubi:OsSAPK6 and ossapk6-2 2-week-old seedlings. Values are means ± SD (n = 3 biological replicates) and the different letters indicate significant differences (P < 0.01) according to Tukey’s honest significant difference (HSD) test. c In vitro cell-free degradation assays showing degradation of GST-IPA1 in extracts from ZH11, Ubi:OsSAPK6, and ossapk6-2 plants in the presence of ATP. d In vitro cell-free degradation assay showing degradation of GST-IPA1 and GST-IPA1S201A213A in the extracts from Ubi:OsSAPK6 plants in the presence of ATP. GST-IPA1 was detected with anti-GST monoclonal antibody and quantitated by densitometry with actin as a control. See also Supplementary Fig. S4.
