Fig. 4: Unbiased clustering reveals a common program of p-EMT in SBC. | Cell Discovery

Fig. 4: Unbiased clustering reveals a common program of p-EMT in SBC.

From: Single-cell transcriptome reveals cellular hierarchies and guides p-EMT-targeted trial in skull base chordoma

Fig. 4: Unbiased clustering reveals a common program of p-EMT in SBC.The alternative text for this image may have been generated using AI.

a Heatmap depicted pairwise correlations of 120 intratumoral programs derived from six patients. Eight meta-signatures reflected intratumoral programs. b Heatmap showed differentially expressed genes (rows) identified by non-negative matrix factorization (NNMF) clustered by their expression across single cells (columns) from a representative tumor (Patient 1). The gene clusters reveal intratumoral programs that are differentially expressed in Patient 1. The corresponding gene signatures were numbered, and selected genes were indicated (right). c The 1,374 cells identified as the Mes-type or the Epi-type were clustered and visualized. d Single-cell trajectories and the variations of gene pseudotime were visualized. e Heatmap showed NNMF gene scores aggregated by samples common (top rows) and tumor-specific (bottom rows) genes within the p-EMT program. f, g Knockdown of TGFBI by siRNA attenuated the invasiveness of UM-chor1. The results of quantification were shown on the right side. S1, S2: two different siRNA. NC: normal control. Scale bar, 400 μm, ***P-value ≤ 0.001. h IHC demonstrated that the expression of ZEB2 was involved in the p-EMT program of SBC.

Back to article page