Fig. 1: SERPINA3 is expressed in VZ/SVZ of human brain.
From: Human SERPINA3 induces neocortical folding and improves cognitive ability in mice
a IF for SERPINA3 and SOX2 in GW15 human brain. Dashed square showed the enlarged part. Scale bars, 50 μm (left), 20 μm (bottom right) and 5 μm (top right). b IF for SERPINA3 and SOX2 in 30 days of brain organoids. Dashed square showed the enlarged part. Scale bars, 100 μm. c RT-qPCR was performed to detect the mRNA level of SERPINA3 in hNPCs and neurons cultured in vitro (n = 6). d–g RT-qPCR was performed to detect the relative mRNA level of PAX6 (n = 6), SOX2 (n = 3), HOPX (n = 4) and SERPINA3 (n = 4) in human ES cells and hNPCs cultured in vitro. h Western-blot analysis for the expression of SERPINA3 in human ES cells and cultured hNPCs in different time from day 1 to day 7. β-actin was detected as loading control. i Statistics of relative intensity of SERPINA3 (n = 3). Two-tail unpaired t-test is used to analyze the data. n.s. no significant difference; P < 0.05 (*), P < 0.01 (**), P < 0.001 (***).
