Fig. 4: FBXW7 promotes CHD6 ubiquitination in a GSK3β-dependent way. | Cell Discovery

Fig. 4: FBXW7 promotes CHD6 ubiquitination in a GSK3β-dependent way.

From: The chromatin remodeler CHD6 promotes colorectal cancer development by regulating TMEM65-mediated mitochondrial dynamics via EGF and Wnt signaling

Fig. 4: FBXW7 promotes CHD6 ubiquitination in a GSK3β-dependent way.The alternative text for this image may have been generated using AI.

a Consensus sequence of the putative GSK3β phosphorylation motif of CHD6 (T2127 and T2131) (top). Representative immunoblots showing CHD6 steady-state expression in HCT116 cells with increased GSK3β overexpression (bottom). b Representative immunoblots showing CHD6 steady-state expression in HCT116 and DLD-1 cells treated with GSK3 inhibitor CHIR-99021 (2 μM) at the indicated time points. c Representative immunoblots showing CHD6 steady-state expression in HCT116 cells transfected with the indicated plasmid in the presence of CHIR-99021 (2 μM). d 293T cells transfected with the indicated plasmids, in the presence or absence of CHIR-99021 (2 μM) treatment, were immunoprecipitated with an anti-Myc antibody and immunoblotted with the indicated antibodies. Threonine-phosphorylated CHD6 was shown. WCL: whole cell lysates. e HCT116 cell lysates were immunoprecipitated (IP) with an anti-CHD6 antibody and immunoblotted with anti-GSK3β antibody. f 293T cells transfected with the indicated plasmids were immunoprecipitated with an anti-Flag antibody and immunoblotted with the indicated antibodies. g 293T cells were transfected with the indicated plasmids and treated with MG132 (10 μM) 6 h before harvest. The cell lysates were pulled down with nickel beads and immunoblotted with the indicated antibodies. h 293T cells were transfected with the indicated plasmids, in the presence or absence of CHIR-99021 treatment. All plates were added with MG132 (10 μM) 6 h before harvest. The cell lysates were pulled down with nickel beads and immunoblotted with an anti-Flag antibody. i 293T cells were transfected with the indicated plasmids. The cell lysates were immunoprecipitated with an anti-Flag antibody and immunoblotted with the indicated antibodies. j 293T cells were transfected with the indicated plasmids and treated with MG132 (10 μM) 6 h before harvest. The cell lysates were pulled down with nickel beads and immunoblotted with the indicated antibodies.

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