Fig. 2: Development of endonuclease facilitated MM-seq method for 6mA mapping in C. reinhardtii and E. coli.
From: High-precision mapping reveals rare N6-deoxyadenosine methylation in the mammalian genome
a T7E1 and MBN endonuclease can recognize and digest 6mA sites in dsDNA oligos after immunoprecipitation. Sanger sequencing traces of the cleavage products are shown below. b A schematic diagram of MM-seq using T7E1 and MBN endonucleases. c Cleavage signals of reads mapped to the known 6mA sites in a genome-wide scale. d Accumulative plot of the distances between the 6mA sites and the transcription start sites (TSS). e Sequence logo showing VATB motif sequences of the 6mA sites detected in C. reinhardtii. f The distribution of the 6mA sites identified in E. coli displays a similar pattern to the genome-wide GATC motif. TSS transcription start site, TTS transcription termination site.
