Fig. 3: Exonuclease facilitated MM-seq and its application in multiple organisms.
From: High-precision mapping reveals rare N6-deoxyadenosine methylation in the mammalian genome
a A fragment resulting from Lambda exonuclease digestion of the synthetic oligo with a 6mA site. Sanger sequencing traces for the cleavage products is shown below. b A schematic diagram showing optimized MM-seq using the Lambda exonuclease. c Nucleotides composition at the 5’ terminal of NGS sequence reads. d Consensus sequences of the genomic context to the 6mA sites identified in C. reinhardtii and E. coli. e High number of overlapping 6mA sites detected between T7E1 endonuclease and Lambda exonuclease in C. reinhardtii and E. coli. f IGV illustration of 6mA sites identified in this study compared to published results from MeDIP-seq and DA-6mA-seq.
