Fig. 2: Lineage tracing of Foxl2-expressing cells in the fetal ovary with Foxl2-CreERT2 mice. | Cell Discovery

Fig. 2: Lineage tracing of Foxl2-expressing cells in the fetal ovary with Foxl2-CreERT2 mice.

From: Dissecting the fate of Foxl2-expressing cells in fetal ovary using lineage tracing and single-cell transcriptomics

Fig. 2: Lineage tracing of Foxl2-expressing cells in the fetal ovary with Foxl2-CreERT2 mice.The alternative text for this image may have been generated using AI.

a No GFP were observed in theca-interstitial cells when Foxl2-CreERT2 mice was induced with tamoxifen at 3 weeks of age. mT/mG; Foxl2-CreERT2 mice were induced with tamoxifen at 3 weeks of age and the GFP signal was examined by immunostaining. No GFP signal was detected in the ovaries of mT/mG mice after tamoxifen induction. GFP signal was only detected in FOXL2-positive granulosa cells (bottom panel, white arrows) of mT/mG; Foxl2-CreERT2 mice and no GFP signal was detected in theca-interstitial cells. b GFP-positive cells were observed as both granulosa cells and theca-interstitial cells of mT/mG; Foxl2-CreERT2 mice when Cre was induced with tamoxifen at E13.5. mT/mG; Foxl2-CreERT2 mice were induced with tamoxifen at E13.5 and GFP signal was examined by immunostaining at 3 weeks of age. No GFP signal was detected in the ovaries of mT/mG mice after tamoxifen induction (top panel). GFP signal was detected in both WT1-positive granulosa cells (inset, white arrows) and WT1-negative theca-interstitial cells (inset, white arrowheads).

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