Fig. 2: Allosteric agonism of GPR35 by cations.
From: Insights into divalent cation regulation and G13-coupling of orphan receptor GPR35
a Architecture of the cation coordination site. The cation is coordinated by the backbone oxygen of G9NT and S11NT, the side chain of R164ECL2, and a carbonyl oxygen atom of lodoxamide. Cation in GPR35 is displayed as a green sphere, while its EM density is colored in grey. Interactions between the cation and surrounding residue coordinates are indicated as blue dashed lines. b–h Allosteric regulatory effects of different cations on lodoxamide-induced G13 recruitment by GPR35, including Mg2+ (b), Ca2+ (c), Mn2+ (d), Co2+, Zn2+, Cu2+ (e), K+ (f), Li+ (g), and Fe3+ (h). The concentrations of different cations are indicated. The physiological concentrations of Co2+, Zn2+, Cu2+, and Fe3+ are much lower than those of Mg2+ and Ca2+ (WHO Vitamin and Mineral Nutrition Information System, VMNIS). The maximum cell safety concentrations of these cations under our experimental conditions were used.
