Fig. 3: Nuclear PD-L1 activates EGR1 transcription.
From: Nuclear PD-L1 promotes EGR1-mediated angiogenesis and accelerates tumorigenesis
a The genomic distribution of PD-L1 CUT&Tag peaks in MUM2B cells. b PD-L1 CUT&Tag signal height and position relative to transcription start sites (TSSs) for all genes in MUM2B cells. Two replicates are shown. c CUT&Tag density heatmap of PD-L1 enrichment in MUM2B cells within 3 kb around TSS. d Bioinformatics analysis identified EGR1 as a downstream target of nPD-L1. e Correlation plot of EGR1 expression and angiogenesis GSVA score in the TCGA-UM cohort. Correlation coefficients were calculated by the Spearman test. f IGV tracks for EGR1 from PD-L1 CUT&Tag analysis. g IGV tracks for EGR1 expression from RNA-seq analysis of PD-L1-silenced cells and control cells. h, i ChIP-qPCR assay of the PD-L1 status at the EGR1 promoter and its upstream and downstream regions after PD-L1 silencing in MUM2B (h) and MEL290 (i) cells. j Western blot images showing the protein levels of EGR1 and VEGFA in PD-L1-silenced cells and control cells. k IF images of EGR1 in UM orthotopic xenografts. Scale bars, 50 µm. l Statistical analysis of EGR1 expression in UM orthotopic xenografts. n = 5. Data are presented as means ± SD. Two-tailed unpaired Student’s t-test.
