Fig. 5: Cluster 2-Mesen_CAF, Macro_APOE/NRG1 and TNFRSF4+ Treg cells were increased from DN to T stage. | Cell Discovery

Fig. 5: Cluster 2-Mesen_CAF, Macro_APOE/NRG1 and TNFRSF4+ Treg cells were increased from DN to T stage.

From: Single-cell and spatial dissection of precancerous lesions underlying the initiation process of oral squamous cell carcinoma

Fig. 5

a Cell proportions of Cluster 2-Mesen_CAF (Cluster 2-Mesen_CAF/CAF) in patients of T stage compared with N + DN stages by scRNA-seq data. b Violin plot displaying Cluster 2 marker gene expressions in Cluster 2-Mesen_CAF, other clusters in Mesen_CAF and Infla_CAF. Two-sided Wilcoxon rank-sum tests for analyzing the significance of their differences. ***P < 0.001. c Statistical results showing proportions of (IGFBP3, POSTN) positive points in different tissue regions by ST analyses. A two-tailed unpaired Student’s t test for the P values. *P < 0.05. d The line charts showing changes of (Macro_APOE/NRG1) cell proportions in each individual from DN to T stage. A two-tailed paired Student’s t test for the P values. *P < 0.05. e Heatmap showing enrichment Macro_APOE/NRG1 and degrees of M1/M2/angiogenesis/glycolysis/ROS pathways by comparison of their marker genes expression levels in different distributions of ST samples. Each row shared a color scale, while different columns did not. f Pearson correlation plot showing significant positive correlation of Macro_APOE/NRG1 proportion to average CTSC expression in the epithelial cells. Cor.test() was used for conducting correlation and the statistical analyses. g Volcano plots showing strikingly DEGs between TNFRSF4+ Tregs and TNFRSF4− Tregs. h Violin plots showing the metagene expression of the IL2-STAT5 signaling in TNFRSF4+ and TNFRSF4− Tregs. P value was calculated by Wilcoxon test. i Cell proportions of TNFRSF4+ Treg cells in patients of diverse initiation stages by scRNA-seq data. j Proportions of cell number ratios of CD8_Tem cells to CD4_TNFRS4+ Treg cells in patients by scRNA-seq data. k ST feature plots of P1 and P2 showing expression of TNFRSF4 in different distributions. l Statistical results showing proportions of TNFRSF4 positive points in different tissue regions by ST analyses (right). m mIHC staining of APOE, CD68, FOXP3, OX40 (TNFRSF4) and panCK in one patient of diverse initiation stages. Scale bars: 500 μm. The numbers in images indicated (CD68+ &APOE+ ; FOXP3+ &OX40+) double-positive cell proportions among corresponding CD68+ and FOXP3+ cells.

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