Fig. 4: SARS-CoV-2 GFP/ΔN infection in EIIA-hACE2-NF/F mice. | Cell Discovery

Fig. 4: SARS-CoV-2 GFP/ΔN infection in EIIA-hACE2-NF/F mice.

From: A tissue specific-infection mouse model of SARS-CoV-2

Fig. 4: SARS-CoV-2 GFP/ΔN infection in EIIA-hACE2-NF/F mice.

a EIIA-hACE2-NF/F mice were intranasally challenged with 1 × 105 TCID50 virus each and sacrificed to collect tissue samples at 7 dpi. b Pathological changes in lungs of EIIA-hACE2-NF/F mice after infection. Five euthanized mice were used to examine the pathological changes in the lungs at 7 dpi. c Immunofluorescence staining of mouse lung paraffin sections against the SARS-CoV-2 GFP/ΔN GFP protein (green), N-HA (red), and DAPI (blue). d Tissue distribution of SARS-CoV-2 viral RNA (the GFP gene RNA) level which relative to the Sftpc-hACE2-N mouse lung tissue group. Each tissue was processed with viral RNA copies analysis by real-time qPCR. e, f Immunofluorescence staining of mouse intestine (e) and stomach (f) paraffin sections for SARS-CoV-2 GFP/ΔN GFP protein (green) and DAPI (blue).

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