Fig. 1: Structure determination of the SIN3L and SIN3S complexes from S. pombe.

a Purification and schematic view of the SIN3L complex. The components of SIN3L were separated on 12% SDS-PAGE and further confirmed by mass spectrometry analysis. The schematic diagram shows the subunit organization of the SIN3L complex. Three components (Cti6, Laf1, and Laf2), present in a purified sample, are labeled in dotted circles for not included in the final model. b Purification and schematic of the SIN3S complex. The components of SIN3S were separated on 12% SDS-PAGE and further confirmed by mass spectrometry analysis. The schematic diagram shows the subunit organization of the SIN3S complex. c Overall structure of the SIN3L complex. The 3.2-Å EM map is shown in the upper panel with individual component color-coded identically. In the lower panel, the Pst1 Lobe (containing Pst1, Clr6, and Prw1) and the Pst3 Lobe (containing Pst3, Clr6’, and Prw1’) are present as a colored cartoon with the whole complex in transparent surface. d Overall structure of the SIN3S complex. The 2.9-Å EM map is shown in the upper panel with individual component color-coded identically. In the lower panel, the Pst2 Lobe (containing Pst2, Clr6, and Prw1) is present as a colored cartoon with the whole complex in transparent surface. All structural images in this paper were generated in ChimeraX⁶⁴ and PyMOL⁶⁵.