Fig. 1: Construction of a cyan SmFP.
From: Design of a palette of SNAP-tag mimics of fluorescent proteins and their use as cell reporters
a Schematic representation of a cyan SmFP (F485-SNAPf complex, SmFP485). The synthetic, non-fluorescent BG-F485 fluorophore became fluorescent once it formed a covalent complex with SNAPf protein. b Absorption spectra of 5 μM BG-F485 (red) or SmFP485 (cyan). c Excitation (dashed) and emission (solid) spectra of 5 μM BG-F485 (red) or SmFP485 (cyan). d Fluorescent images of SmFP485-expressing cells. HeLa cells transfected with or without pcDNA3.1-SNAPf vector, and imaged after incubation with 2 μM BG-F485. Scale bars, 10 μm. e Images of SmFP485 fluorescence generation in sequential frames in cells expressing plasma membrane-localized SNAPf. HeLa cells co-transfected with pDisplay-SNAPf and pDisplay-mCherry were imaged after incubation with 2 μM BG-F485. Scale bars, 20 μm. f Fluorogenic labeling of subcellular targeted SNAPf fusions recorded without washing. Scale bars, 10 μm.
