Fig. 3: Class I HDAC 1/2/3 are the major histone desuccinylases in mammalian cells.
From: HDAC1/2/3 are major histone desuccinylases critical for promoter desuccinylation
a WB analysis showing the effect of single KO of HDAC1, HDAC2, or HDAC3 on histone succinylation in HeLa cells. KO of each HDAC was conducted by two different sgRNAs. b WB analysis showing histone succinylation in HeLa, HCT116, and MCF7 cells with a combinatorial KO of HDAC1/2/3. c WB analysis showing histone succinylation in HeLa, HCT116, and MCF7 cells with combinatorial KD of HDAC1/2/3 by siRNAs. Cells were treated with siRNAs for 72 h. d WB analysis showing the effect of ectopic expression of wild-type HDAC1, HDAC2, or HDAC3 and their corresponding HDAC activity-deficient mutants in two breast cancer cell lines SUM159 (left) and MDA-MB468 (right).
