Fig. 2: Single-cell RNA-sequencing analyses identify the adherent leukocytes on schistosomes in M. fortis.

a Overview of the scRNA-seq analyses, illustrating the isolation and scRNA-seq analysis of the leukocytes adhered to the surface of schistosomes in M. fortis. b UMAP maps indicate the expression of canonical macrophage markers. Data are shown as normalized transcript counts on a color-coded logarithmic scale. c Gene set enrichment analysis of the top 200 highly expressed genes. d Heatmap showing the regulons (rows) activity in each cell (columns) as estimated by SCENIC. For each regulon, the transcription factor and the number of predicted target genes are indicated. The gene name suffix (_extended) represents that the reliability of the annotated target gene is relatively low.