Fig. 1: Cryo-EM structures of the FZD1-Gq, FZD3-Gs and FZD6-Gs complexes.
From: A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways
The BRET-based Gq and Gs biosensor is used to examine the constitutive activity of FZD1, FZD3 and FZD6 (left). Cryo-EM map (middle) and cartoon representation of the atomic model (right) of the FZD1-Gq (a), FZD3-Gs (b) and FZD6-Gs (c) complexes were shown, respectively. Color coding is annotated for each protein component. BRET ratios are computed as the ratio of the GFP2 emission to RLuc8 emission (see Materials and methods). Data are mean ± s.e.m. (n = 4). Cryo-EM maps of FZD1 and FZD6 are automatically sharpened by deepEMhancer. The cryo-EM map of FZD3 is sharpened by a global B-factor (see Materials and methods).
