Fig. 3: Molecular switch for the activation of FZDs.
From: A framework for Frizzled-G protein coupling and implications to the PCP signaling pathways
Superposition of the inactive and active FZD1 (a), FZD3 (b) and FZD6 (c) structures. d Conformational rearrangement of the R6.32-W7.55 molecular switch of SMO (inactive, 4JKV; active, 6OT0), FZD1, FZD3 and FZD6 upon G protein coupling are indicated by red arrows. Color coding is annotated for each protein component. e The combined mutations of R6.32A and W7.55A reduced FZD1 (left), FZD3 (middle) and FZD6 (right) mediated G protein signaling. ΔBRET represents the change of bioluminescence resonance energy transfer value. Significance was determined by two-way ANOVA with Two-stage Benjamini, Krieger, & Yekutieli FDR procedure (*P < 0.05; **P < 0.01). Data are mean ± s.e.m. (n ≥ 3).
