Fig. 3: Construction of INSRTR protein logic gates based on intramolecular fusion with interacting and protease target segments. | Cell Discovery

Fig. 3: Construction of INSRTR protein logic gates based on intramolecular fusion with interacting and protease target segments.

From: Designed allosteric protein logic

Fig. 3

a Scheme of the implementation of INSRTR logic gates with protease inputs and allosterically activated protein function. b Schematic presentation of an AND logic gate. c Experimental testing of all nontrivial two-input Boolean logic functions by INSRTR in HEK293T cells, as regulated by a combination of PPV and SbMV protease inputs. The values in (c) are the means of four cell cultures ± SD and are representative of at least two independent experiments. Significance was tested with one-way ANOVA with Tukey’s comparison between indicated ON and OFF states. P values 0.1234 (ns), 0.0332 (*), 0.0021 (**), 0.0002 (***), < 0.0001 (****) (significance, confidence intervals, degrees of freedom, F and P values are listed in Supplementary Table S2).

Back to article page