Fig. 3: BCAR3 methylation promotes cell migration and invasiveness.

a, b Representative single-cell migration tracks monitored by time-lapse live microscopy (a) and quantification of single-cell migration velocity (b) of MDA-MB-231 cells with indicated engineering. Boxes: 25th to 75th percentile, whiskers: 10 to 90 percentile, center line: median. P-values were calculated by ANOVA with Tukey’s testing for multiple comparisons. c, d Representative images of three-dimensional collagen I matrix invasion (c) and spreading quantification (d) of basement membrane enclosed MDA-MB-231 with indicated engineering. P-values were calculated by ANOVA with Tukey’s testing for multiple comparisons. Scale bars, 50 μm. e 3D image reconstruction of spheroid edge depicting engineered MDA-MB-231 cells protrusions, related to (c). Scale bars represent 10 µm. In all box plots, the center line indicates the median, the box marks the 75th and 25th percentiles and the whiskers indicate 10 to 90 percentile values.