Fig. 5: Clathrin-mediated pre-Ω and fs-Ω pore closure underlie slow, fast, ultrafast and overshoot endocytosis in chromaffin cells.
From: Clathrin mediates membrane fission and budding by constricting membrane pores
a ICa and Cm (mean ± s.e.m.) induced by depol1s (gray triangle) in three conditions: (1) si-Ctrl (27 cells) or si-CHC (15 cells); (2) sh-Ctrl (16 cells) or sh-CHC (16 cells); and (3) control (Ctrl, 14 cells) or pitstop 2 (PST2, 30 μM, bath, 15–30 min, 13 cells). b Mean ICa and Cm (± s.e.m.) induced by depol1s for Groupno-endo, Groupslow, Groupfast, Groupultrafast and Groupovershoot in si-Ctrl (black, n = 5–6 cells for each group), and for 5 groups in si-CHC (red, 5–6 cells for each group), which were divided based on ICa density similar to 5 si-Ctrl groups (< 22, 22–32, 32–36, 36–41, > 41 pA/pF). c Cm (black, left Y-axis) and Rfs+pre (green, right Y-axis) for 5 individual cells transfected with si-Ctrl showing no-endocytosis, slow, fast, ultrafast or overshoot endocytosis (depol1s: gray triangle). Rfs+pre: the summed number of pre-spot-close and close-fusion (vesicle size corrected, see Methods). d Similar to panel (c), but for si-CHC (cells selected based on ICa density described in b). e Left: Cm decay amplitude (Decay Amp, upper) and Cm decay τ (lower) plotted vs ICa density (mean ± s.e.m) for five groups (each group: 3–6 cells) of cells in si-CHC, sh-CHC, pitstop 2 (PST2) or in control (Ctrlpool, control data pooled from si-Ctrl, sh-Ctrl and Ctrl). Decay Amp normalized to ΔCm; cell grouping is described in c. Right: similar to left but replacing Cm with Rfs+pre (cell groups same as in left).
