Fig. 2: Molecular basis of MCH recognition by MCHR1.
From: Mechanisms of ligand recognition and activation of melanin-concentrating hormone receptors
a Cross-section of the MCH-binding pocket in MCHR1. b‒d Detailed molecular interactions between MCH (depicted in medium purple) and MCHR1 (shown in light salmon). Hydrogen bonds are presented with gray dashed lines. e Functional implications of mutations within the MCHR1 binding pocket, represented as ΔpEC50 values. ΔpEC50 represents the difference in pEC50 values between the WT and the mutants of MCHR1. Data from three independent experiments, each of which was performed in triplicate, are presented as mean ± SEM. Statistical differences were determined by two-sided one-way ANOVA with Tukey’s test compared with WT. NA no activity; NS no significant difference; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
