Fig. 4: DNA unwinding by the truncated MPXV E5 proteins.
From: Structural and functional insights into the helicase protein E5 of Mpox virus
a Domain architectures of the truncated MPXV E5 proteins. b In vitro DNA unwinding assays catalyzed by the N-terminal truncated MPXV E5 (E5_∆N). c The overall folding and assembly of the E5_∆N-ssDNA-AMPPNP complex. The bound DNA is shown as spheres. d Superposition of DNA and the SF3 helicase domains in the E5_∆ N-ssDNA-AMPPNP complex and the E5-ssDNA-AMPPNP structure. MPXV E5, and the bound AMPPNP are all colored in gray in the MPXV E5/DNA structure. e In vitro DNA unwinding assays catalyzed by MPXV E5 with both N- and C-terminal truncation (E5_∆NC).
