Fig. 6: Characterization of the AEP domain of MPXV E5.
From: Structural and functional insights into the helicase protein E5 of Mpox virus
a In vitro primer extension assays catalyzed by the full-length and truncated MPXV E5 proteins. b Overall folding of MPXV E5 AEP domain. c, d Mg2+ coordination and PPi recognition in the MPXV E5 AEP domain structure. e Superposition of the AEP domains of MPXV E5 and VACV D5. f Catalytic site comparison of MPXV E5 AEP domain with the MsCAPP structure. g In vitro primer extension assays catalyzed by WT MPXV E5 and mutant proteins. MPXV E5 AEP domain and the bound Mg2+ are colored in blue-and-yellow and black in b–f.
