Fig. 4: Integrated scRNA-seq analysis of porcine blastoids and embryos.
From: Pig blastocyst-like structure models from embryonic stem cells
a PCA of porcine blastoids and embryos25. EB (early blastula, E6), LB (late blastula, E7), HB (hatched blastula, E8), EBi (early bilaminar embryo, E9), LBi (late bilaminar embryo, E10), PPS (pre-primitive streak embryo, E11), EPS (early primitive streak embryo, E12), PS (primitive streak embryo, E13), and LPS (late primitive streak embryo, E14). b PCA of LB, HB, and EBi. c PCA analysis showing that porcine embryos25 at E7–E9 included five clusters. d Expression scores of EPI (POU5F1 and SOX2), TE (GATA2 and GATA3), and HYPO (GATA4 and GATA6). e Cells derived from porcine embryos are colored by origin: EPI (yellow), HYPO (purple), and TE (red). f UMAP plot of porcine blastoid cells integrated with published porcine embryo cells25 and a pie chart showing the percentages of three lineage cells. The percentages 85.2%/59.4%/60.9% are calculated by dividing the number of EPI/HYPO/TE lineage cells by the number of embryo-derived cells in zone I/II/III, respectively. Similarly, the percentages 76.4%/97.7%/97.8% are calculated by dividing the number of ELCs/HLCs/TLCs by the number of blastoid-derived cells in zone I/II/III, respectively.
